Description

CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

References
1. EMBO J. 1998. 16: 545-554. 2. Science 1998. 282: 1893-1897.

Specification
Aliases	CHEK1
Entrez GeneID	1111
Swissprot	O14757
clone	2G1D5
WB Predicted band size	56kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Purified recombinant fragment of CHK1 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Application
WB	1/500 - 1/2000
ELISA	1/10000