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Western blot analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.
Figure3: Confocal Immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of Hela cells using WNT1 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human LAdrenal tissues using WNT1 mouse mAb
HOT

Mouse Monoclonal Antibody to WNT1

  • 货号:

    20378
  • 别名:

    INT1
  • 应用:

    WB,IHC,ICC,FCM
  • 反应种属:

    Human,Mouse
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to WNT1

Description
WNT1: wingless-type MMTV integration site family, member 1. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant rolein Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
References
1. Blood. 2008 Jan 1;111(1):122-31.
2. BMC Cancer. 2005 May 24;5:53.
Specification
Aliases INT1
Entrez GeneID 7471
Swissprot P04628
clone 10C8
WB Predicted band size 41kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse
Immunogen Purified recombinant fragment of WNT1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000