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All lanes : Anti-PDHA1 Antibody (Center) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Rabbit Polyclonal Antibody to Zebrafish PDHA1

  • 货号:

    P34234
  • 别名:

    PDHE1-A type I; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A, PDHA1;pdha1a;pdha1b
  • 应用:

    WB
  • 反应种属:

    zebrafish
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to Zebrafish PDHA1

Description
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
Specification
Aliases PDHE1-A type I; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A, PDHA1;pdha1a;pdha1b
Entrez GeneID 406702
Swissprot Q6P948
WB Predicted band size 43.7kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity zebrafish
Immunogen This Zebrafish PDHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233~263 amino acids from the Central region of Zebrafish PDHA1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P34234