Description

Thioredoxin (TRX) is a small ubiquitous protein (MW12kDa) which is exist in a wide variety of prokaryotic and eukaryotic cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. This antibody is suitable for detecting fusion proteins which encode a Trx-Tag by immunoblotting and immunoprecipitation. The Monoclonal Antibody can detect a little Trx-Tag fusion proteins with negligible cross-reactivity with bacterial, insect, or mammalian lysates.

References
1. Holmgen, A. et al., Annu. Rev. Biochem. 54, 237-271 (1985). 2. Wollman, E. E. et al., J. Biol. Chem. 263, 15506-15512 (1988). 3. Sasada, T. et al., J. Toxicol. Sci. 21, 285-287 (1996).

Specification
Aliases	Thioredoxin
clone	1H6H6
WB Predicted band size	12kDa
Host/Isotype	Mouse IgG1
Antibody Type	Tag Antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Tag
Immunogen	Purified recombinant fusion protein with Thioredoxin (TRX) tag.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Application
WB	1/500 - 1/2000
IHC	1/100 - 1/1000
ICC	1/100-1/200
FCM	1/200 - 1/400
ELISA	1/10000