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HLA-F

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)</p>
  • <p>Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
  • <p>Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse  Liver (1) tissue lysate.</p>
  • <p>Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).</p>

图片

  • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)

  • Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.

  • Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) tissue lysate.

  • Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).

HLA-F
Catalog#   :  32261
Size/Concentration   :  1.0mg/ml
Price:
¥ 2557.5
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1
Instruction:
Specification
Entrez GeneID3134
AliasesHLAF; CDA12; HLA-5.4; HLA-CDA12
Clone#3F11F4
MW39kDa
Host/IsotypeMouse IgG2b
Storage4℃; -20℃ for long term storage
Species ReactivityHuman; Mouse;
ImmunogenPurified recombinant fragment of human HLA-F (AA: 22-305) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
FCM1/200 - 1/400
Description
This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.
References
1.Hum Reprod. 2020 Mar 27;35(3):705-717.
2.Int J Mol Sci. 2019 Nov 8;20(22):5572.
Sequence

extra(22-305)


Diagram
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)

Western Blot

Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.

 

Western Blot

Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) tissue lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).

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