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BRAF

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)</p>
  • <p>Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.</p><br />
  • <p>Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).</p>
  • <p>Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.</p>
  • <p>Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.</p>

图片

  • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)

  • Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.


  • Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

  • Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.

  • Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.

BRAF
Catalog#   :  32150
Size/Concentration   :  1.0mg/ml
Price:
¥ 2557.5
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1
Instruction:
Specification
Entrez GeneID673
AliasesNS7; B-raf; BRAF1; RAFB1; B-RAF1
Clone#2A10D7
MW84.4kda
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage
Species ReactivityHuman; Rat;
ImmunogenPurified recombinant fragment of human BRAF (AA: 299-447) expressed in HEK293-6e.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
References
1,Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31105-31113.
2,Sci Rep. 2020 Oct 9;10(1):16943.
Sequence

299-447


Diagram
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)

Western Blot

Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.

 

Flow cytometric

Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.

 

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