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GZMK

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 2:Western blot analysis using GZMK mAb against human GZMK (AA: 27-264) recombinant protein. (Expected MW is 28.7kDa)</p>
  • <p>Figure 3:Western blot analysis using GZMK mAb against HEK293-6e (1) and GZMK (AA: 27-264)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
  • <p>Figure 4:Flow cytometric analysis of Jurkat cells using GZMK mouse mAb (green) and negative control (red).</p>
  • <p>Figure 5:Flow cytometric analysis of MOLT4 cells using GZMK mouse mAb (green) and negative control (red).</p>
  • <p>Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using GZMK mouse mAb with DAB staining.</p>

图片

  • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Figure 2:Western blot analysis using GZMK mAb against human GZMK (AA: 27-264) recombinant protein. (Expected MW is 28.7kDa)

  • Figure 3:Western blot analysis using GZMK mAb against HEK293-6e (1) and GZMK (AA: 27-264)-hIgGFc transfected HEK293-6e (2) cell lysate.

  • Figure 4:Flow cytometric analysis of Jurkat cells using GZMK mouse mAb (green) and negative control (red).

  • Figure 5:Flow cytometric analysis of MOLT4 cells using GZMK mouse mAb (green) and negative control (red).

  • Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using GZMK mouse mAb with DAB staining.

GZMK
Catalog#   :  32147
Size/Concentration   :  1.0mg/ml
Price:
¥ 2557.5
浏览量:
1
Instruction:
Specification
Entrez GeneID3003
AliasesTRYP2
Clone#5B4A5
MW29kDa
Host/IsotypeMouse IgG2b
Storage4℃; -20℃ for long term storage
Species ReactivityHuman;
ImmunogenPurified recombinant fragment of human GZMK (AA: 27-264) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description
This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes.
References
1,Brain. 2019 Nov 1;142(11):3411-3427.
2,J Invest Dermatol. 2019 Apr;139(4):930-939.
Sequence

27-264


Diagram
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using GZMK mAb against human GZMK (AA: 27-264) recombinant protein. (Expected MW is 28.7kDa)

Western Blot

Figure 3:Western blot analysis using GZMK mAb against HEK293-6e (1) and GZMK (AA: 27-264)-hIgGFc transfected HEK293-6e (2) cell lysate.

 

Flow cytometric

Figure 4:Flow cytometric analysis of Jurkat cells using GZMK mouse mAb (green) and negative control (red).

Flow cytometric

Figure 5:Flow cytometric analysis of MOLT4 cells using GZMK mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using GZMK mouse mAb with DAB staining.

 

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