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CD109

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)</p>
  • <p>Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
  • <p>Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)</p>
  • <p>Figure 5:Flow cytometric analysis of Jurkat cells using CD109 mouse mAb (green) and negative control (red).</p>
  • <p>Figure 6:Immunohistochemical analysis of paraffin-embedded lung tissues using CD109 mouse mAb with DAB staining.</p>
  • <p>Figure 7:Immunohistochemical analysis of paraffin-embedded stomach tissues using CD109 mouse mAb with DAB staining.</p>

图片

  • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)

  • Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.

  • Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • Figure 5:Flow cytometric analysis of Jurkat cells using CD109 mouse mAb (green) and negative control (red).

  • Figure 6:Immunohistochemical analysis of paraffin-embedded lung tissues using CD109 mouse mAb with DAB staining.

  • Figure 7:Immunohistochemical analysis of paraffin-embedded stomach tissues using CD109 mouse mAb with DAB staining.

CD109
Catalog#   :  32141
Size/Concentration   :  1.0mg/ml
Price:
¥ 2557.5
浏览量:
1
Instruction:
Specification
Entrez GeneID135228
Aliasesp180; r150; CPAMD7
Clone#3B5G6
MW161.7kDa
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage
Species ReactivityHuman;
ImmunogenPurified recombinant fragment of human CD109 (AA: extra 1274-1421) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/20 - 1/100
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description
This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.
References
1.Cancer Sci. 2020 May;111(5):1652-1662.
2.Diagn Pathol. 2015 Aug 7;10:137.
Sequence

extra(1274-1421)


Diagram
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)

Western Blot

Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.

 

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 5:Flow cytometric analysis of Jurkat cells using CD109 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded lung tissues using CD109 mouse mAb with DAB staining.

 

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded stomach tissues using CD109 mouse mAb with DAB staining.

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