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CEACAM6

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 2:Western blot analysis using CEACAM6 mAb against human CEACAM6 (AA: 35-256) recombinant protein. (Expected MW is 55 kDa)</p>
  • <p>Figure 3:Western blot analysis using CEACAM6 mouse mAb against Jurkat (1), PC-12 (2), HEK293 (3), MG63 (4), and C2C12 (5) cell lysate.</p>
  • <p>Figure 4:Immunofluorescence analysis of Hela cells using CEACAM6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)</p>
  • <p>Figure 5:Flow cytometric analysis of Jurkat cells using CEACAM6 mouse mAb (green) and negative control (red).</p>
  • <p>Figure 6:Immunohistochemical analysis of paraffin-embedded colon tissues using CEACAM6 mouse mAb with DAB staining.</p>
  • <p>Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CEACAM6 mouse mAb with DAB staining.</p>

图片

  • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Figure 2:Western blot analysis using CEACAM6 mAb against human CEACAM6 (AA: 35-256) recombinant protein. (Expected MW is 55 kDa)

  • Figure 3:Western blot analysis using CEACAM6 mouse mAb against Jurkat (1), PC-12 (2), HEK293 (3), MG63 (4), and C2C12 (5) cell lysate.

  • Figure 4:Immunofluorescence analysis of Hela cells using CEACAM6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • Figure 5:Flow cytometric analysis of Jurkat cells using CEACAM6 mouse mAb (green) and negative control (red).

  • Figure 6:Immunohistochemical analysis of paraffin-embedded colon tissues using CEACAM6 mouse mAb with DAB staining.

  • Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CEACAM6 mouse mAb with DAB staining.

CEACAM6
Catalog#   :  32139
Size/Concentration   :  1.0mg/ml
Price:
¥ 2557.5
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1
Instruction:
Specification
Entrez GeneID4680
AliasesNCA; CEAL; CD66c
Clone#3H4A5
MW37.2kDa
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage
Species ReactivityHuman; Mouse; Rat;
ImmunogenPurified recombinant fragment of human CEACAM6 (AA: 35-256) expressed in HEK293-6e cells supernatant.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/25 - 1/100
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description
This gene encodes a protein that belongs to the carcinoembryonic antigen (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. Members of this family play a role in cell adhesion and are widely used as tumor markers in serum immunoassay determinations of carcinoma. This gene affects the sensitivity of tumor cells to adenovirus infection. The protein encoded by this gene acts as a receptor for adherent-invasive E. coli adhesion to the surface of ileal epithelial cells in patients with Crohn's disease. This gene is clustered with genes and pseudogenes of the cell adhesion molecules subgroup of the CEA family on chromosome 19.
References
1.Sci Rep. 2019 Dec 4;9(1):18347.
2.J Immunol. 2017 Apr 15;198(8):3307-3317.
Sequence

35-256


Diagram
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CEACAM6 mAb against human CEACAM6 (AA: 35-256) recombinant protein. (Expected MW is 55 kDa)

Western Blot

Figure 3:Western blot analysis using CEACAM6 mouse mAb against Jurkat (1), PC-12 (2), HEK293 (3), MG63 (4), and C2C12 (5) cell lysate.

 

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CEACAM6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 5:Flow cytometric analysis of Jurkat cells using CEACAM6 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded colon tissues using CEACAM6 mouse mAb with DAB staining.

 

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CEACAM6 mouse mAb with DAB staining.

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