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CD178

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 4:Western blot analysis using CD178 mAb against human CD178 (AA: extra 103-281) recombinant protein. (Expected MW is 46.4 kDa)</p>
  • <p>Figure 5:Western blot analysis using CD178 mAb against HEK293-6e (1) and CD178 (AA: extra 103-281)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
  • <p>Figure 4:Immunofluorescence analysis of Hela cells using CD178 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)</p>
  • <p>Figure 5:Flow cytometric analysis of Jurkat cells using CD178 mouse mAb (green) and negative control (red).</p>

图片

  • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Figure 4:Western blot analysis using CD178 mAb against human CD178 (AA: extra 103-281) recombinant protein. (Expected MW is 46.4 kDa)

  • Figure 5:Western blot analysis using CD178 mAb against HEK293-6e (1) and CD178 (AA: extra 103-281)-hIgGFc transfected HEK293-6e (2) cell lysate.

  • Figure 4:Immunofluorescence analysis of Hela cells using CD178 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • Figure 5:Flow cytometric analysis of Jurkat cells using CD178 mouse mAb (green) and negative control (red).

CD178
Catalog#   :  32136
Size/Concentration   :  1.0mg/ml
Price:
¥ 2557.5
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1
Instruction:
Specification
Entrez GeneID356
AliasesFASLG; APTL; FASL; CD95L; ALPS1B; CD95-L; TNFSF6; TNLG1A; APT1LG1
Clone#2A6E12
MW31.5kDa
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage
Species ReactivityHuman;
ImmunogenPurified recombinant fragment of human CD178 (AA: extra 103-281) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/50 - 1/200
FCM1/200 - 1/400
Description
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described.
References
1.Mol Cancer Res. 2019 Dec;17(12):2537-2548.
2.Cell Death Dis. 2018 Jun 11;9(6):695.
Sequence

extra(103-281)


Diagram
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 4:Western blot analysis using CD178 mAb against human CD178 (AA: extra 103-281) recombinant protein. (Expected MW is 46.4 kDa)

Western Blot

Figure 5:Western blot analysis using CD178 mAb against HEK293-6e (1) and CD178 (AA: extra 103-281)-hIgGFc transfected HEK293-6e (2) cell lysate.

 

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CD178 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 5:Flow cytometric analysis of Jurkat cells using CD178 mouse mAb (green) and negative control (red).

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