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KIT

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 2:Western blot analysis using KIT mAb against human KIT (AA: 23-322) recombinant protein. (Expected MW is 59.6 kDa)</p>
  • <p>Figure 3:Western blot analysis using KIT mAb against HEK293-6e (1) and KIT (AA: 23-322)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
  • <p>Figure 4:Immunofluorescence analysis of Hela cells using KIT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)</p>
  • <p>Figure 5:Flow cytometric analysis of K562 cells using KIT mouse mAb (green) and negative control (red).</p>

图片

  • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Figure 2:Western blot analysis using KIT mAb against human KIT (AA: 23-322) recombinant protein. (Expected MW is 59.6 kDa)

  • Figure 3:Western blot analysis using KIT mAb against HEK293-6e (1) and KIT (AA: 23-322)-hIgGFc transfected HEK293-6e (2) cell lysate.

  • Figure 4:Immunofluorescence analysis of Hela cells using KIT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • Figure 5:Flow cytometric analysis of K562 cells using KIT mouse mAb (green) and negative control (red).

KIT
Catalog#   :  32134
Size/Concentration   :  1.0mg/ml
Price:
¥ 2557.5
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1
Instruction:
Specification
Entrez GeneID3815
AliasesPBT; SCFR; C-Kit; CD117; MASTC
Clone#1H1B1
MW110kDa
Host/IsotypeMouse IgG2b
Storage4℃; -20℃ for long term storage
Species ReactivityHuman;
ImmunogenPurified recombinant fragment of human KIT (AA: 23-322) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/50 - 1/200
FCM1/200 - 1/400
Description
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
References
1.Genes Chromosomes Cancer. 2019 Nov;58(11):798-803.
2.Exp Hematol. 2019 Jun;74:19-24.e4.
Sequence

23-322


Diagram
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KIT mAb against human KIT (AA: 23-322) recombinant protein. (Expected MW is 59.6 kDa)

Western Blot

Figure 3:Western blot analysis using KIT mAb against HEK293-6e (1) and KIT (AA: 23-322)-hIgGFc transfected HEK293-6e (2) cell lysate.

 

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using KIT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 5:Flow cytometric analysis of K562 cells using KIT mouse mAb (green) and negative control (red).

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