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ATP1A1

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
  • <p>Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)</p>
  • <p>Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
  • <p>Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.</p>
  • <p>Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)</p>
  • <p>Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).</p>
  • <p>Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP1A1 mouse mAb with DAB staining.</p>
  • <p>Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.</p>

图片

  • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)

  • Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.

  • Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.

  • Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).

  • Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP1A1 mouse mAb with DAB staining.

  • Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.

ATP1A1
Catalog#   :  32106
Size/Concentration   :  1.0mg/ml
Price:
¥ 2557.5
浏览量:
1
Instruction:
Specification
Entrez GeneID476
AliasesCMT2DD; HOMGSMR2
Clone#3C1F3
MW112.8kDa
Host/IsotypeMouse IgG2a
Storage4℃; -20℃ for long term storage
Species ReactivityHuman;
ImmunogenPurified recombinant fragment of human ATP1A1 (AA: 153-288) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/200 - 1/1000
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
References
1.Autophagy. 2018;14(8):1359-1375.
2.Sci Rep. 2016 Jun 14;6:27738.
Sequence

153-288


Diagram
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)

Western Blot

Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.

 

Western Blot

Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).

 

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP1A1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.

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